Since we showed that blocking of autophagy by genetic (ATG5 siRNA and ATG7 siRNA) or pharmacological (CQ) means induced cell death in GBC cells grown with 5-FU, it’s possible that autophagy plays a protective role in proteasome inhibitor-induced cell death by elimination cytotoxic cellular component, it may be an resistant factor which diminishes therapeutic effect in both sensitivities and resistantance of gallbladder carcinoma. Here, ATG5 is linked to gallbladder carcinoma.