To define whether EZH2 was required to sustain embryonal RMS proliferation, as it occurs for other kind of human cancers [36,37], cell proliferation of the established embryonal RMS cell line RD, derived from a tumor recurrence [38], and cultured in growth medium, i.e. supplemented with 10% serum, was evaluated upon EZH2 genetic silencing. Here, EZH2 is linked to cancer.