Additionally, several colorectal biomarkers, including TS, DPD (18) and vascular endothelial growth factor (VEGF) (30) and most ColoPrint reference genes (8), including MCTP1, CTSC and PYROXD1, were in group B, indicating the reliability and reproducibility of these genes for molecular analyses in colorectal cancer. The gene discussed is CTSC; the disease is colorectal cancer.