Additionally, several colorectal biomarkers, including TS, DPD (18) and vascular endothelial growth factor (VEGF) (30) and most ColoPrint reference genes (8), including MCTP1, CTSC and PYROXD1, were in group B, indicating the reliability and reproducibility of these genes for molecular analyses in colorectal cancer. This evidence concerns the gene PYROXD1 and colorectal cancer.