After 20 days, we dissociated skin-draining lymph nodes and re-stimulated lymph node lymphocytes with Her2/neu peptides, mitomycin C-treated BR5FVB1 tumor cells, or BR5FVB1 tumor cell lysate, and performed flow cytometric analysis for the presence of Granzyme B-generating CD4+ and CD8+ T cells. Here, GZMB is linked to neoplasm.