Therefore, combining the microarray and qPCR results, upregulation of E2F6, HMGA1, IRF1, and TFDP1 and downregulation or no expression of SUV39H1, RBL1, HNRPD can be used as diagnostic markers of NSCLC, and, in particular, adenocarcinoma and squamous cell carcinoma. The gene discussed is HNRNPD; the disease is squamous cell carcinoma.