PLAT and cardiovascular disorder: Thus, up-regulation and activation of PKC are key upstream events required for synthesis of PGI2. Our qRT-PCR and Western blotting results demonstrated that expression of PKC increased in both HUVEC-PGI2S and HUVEC-PGI2S-tPA cells, PKC has been reported to be widely expressed in various types of tissues and plays a role in cardiovascular disease.