INS and hepatocellular carcinoma: On the basis of the enzymatic inhibition results, Daiokanzoto, Masiningan, Tokakujokito, Keimakakuhanto and Choijokito were further evaluated for their cellular activity in the insulin-signal-transduction pathway in the human hepatocellular carcinoma cell line, HepG2, by measuring the phosphorylation level of Akt, a key downstream effector of the insulin-signaling cascade.