These results strongly suggest that TMZ-mediated stimulation of the WNK1/OSR1/NKCC1 cascade has dual effects on glioma, it counteracts against loss of K+i, Cl-i and AVD in order to promote GC survival, and it also functions to maintain focal cell volume regulation and facilitates glioma migration. The gene discussed is SLC12A2; the disease is central nervous system cancer.