MYC and prostate cancer: To perform CLSM studies, DU145 human prostate cancer cells were incubated with the c-myc-antisense-Gd@BioShuttle complex labeled with an Alexa Fluor® 546-conjugated fluorescent dye at the non-cleavable lysine-spacer site on the ε-amino group.[22] In Figure 3, the intracellular localization of the c-myc-antisense-Gd@BioShuttle transporter after 5, 10, 20 and 30 min incubation time is illustrated via clear cytoplasmic fluorescence signals.