To determine if SEMA7A plays a role in monocyte migration, a modified Boyden chamber assay was performed using RAW 264.7 murine macrophages and conditioned media from wild type DA-3 tumor, DA-3 scramble shRNA, or DA-3 SEMA7A shRNA knockdown cells as possible chemoattractants. Here, SEMA7A is linked to neoplasm.