4–5 weeks post-tumor cell implantation, thioglycollate elicited macrophages from DA-3 scramble shRNA control or DA-3 SEMA7A shRNA mammary tumor-bearing mice were analyzed for the production of pro-angiogenic chemokines CXCL2/MIP-2, CXCL1 and matrix metalloprotease MMP-9. The gene discussed is CXCL2; the disease is breast cancer.