Lumachi et al. used simple multianalyte immunoassay for measurement of five markers, CEA, CA 19-9 and 72-4, CYFRA 21-1, and osteopontin, comparing their expressions in patients with colorectal cancer and age- and sex-matched patients suffering from confirmed benign colorectal diseases (controls) [175]. This evidence concerns the gene CEACAM5 and colorectal cancer.