We assessed whether the GATA3 and GATA5 qMSP assays are capable of methylation detection in normal human primary tubule epithelial cells and in cancer cell lines, each respectively used as a proxy for normal tissues and localized and metastatic human cancers of other origin (kidney, prostate, bladder, breast and cervical cancer cell lines), which in part have already been reported to demonstrate tumor specific hypermethylation. Here, GATA3 is linked to cancer.