Although aberrant Notch receptor expression has been reported in osteosarcoma [10] and manipulation of the Notch pathway has been shown to play a crucial role for Hes1 (a Notch-dependent gene) in osteosarcoma invasion and metastasis [11,12], whether the ADAM10–Notch1 signaling axis is particularly involved in osteosarcoma is unknown. Here, HES1 is linked to osteosarcoma.