Subgroup analysis was performed to evaluate whether the pooled estimates of OS, DFS, tumor grade, TNM stage, vascular invasion, tumor size ≥ 5 cm and tumor multifocality were different according to the GPC3 cut-off values of 0%, 10%, 20%, and 25% of positive tumor cells by IHC staining reported in the included studies. Here, GPC3 is linked to neoplasm.