Nevertheless, in PIK3CA wild type group, PIK3CA amplification was detected in 22(20.4%, 20/108) tumors and was significantly associated with male gender (22/90 vs. 0/18, p = 0.021), current/former smoker (22/78 vs. 0/30, p = 0.001) and squamous cell carcinoma pathological type (19/52 vs. 3/56, p<0.0001). The gene discussed is PIK3CA; the disease is squamous cell carcinoma.