To examine whether NF-κB activated transcription from the promoter of human Mcl-1 gene in Mcl-1-expressing ESCC cell lines, different series of human esophageal carcinoma cell lines TE-1, Eca109 and KYSE150 were transiently transfected with the luciferase reporter plasmid containing a 325 bp long human Mcl-1 promoter fragment. This evidence concerns the gene NFKB1 and carcinoma of esophagus.