To confirm such differential cytological locations of TSPY in both clinical samples and LADY model of prostate cancer, we have examined the TSPY expression with reference to the forkhead class DNA-binding protein A1 (FOXA1) biomarker, which is preferentially expressed in prostatic epithelial cells and play crucial roles in prostate cancer initiation and development in both human specimens and mouse models [48-50]. The gene discussed is FOXA1; the disease is Familial prostate cancer.