However, only one study have reported that the miR-34a was silenced in ESCC cell lines and re-expression miR-34a can inhibit the ESCC proliferation by reducing the C-met and Cyclin D1 expression [24], yet the correlation between downregulation/loss of miR-34a expression and promoter methylation in ESCC was not clean, especially in the Kazakh population. The gene discussed is CCND1; the disease is esophageal squamous cell carcinoma.