Indeed, they reported that the downregulation of miR-21 in colon cancer cell lines (HCT-116 or HT-29), through transfection of antisense miR-21, induced differentiation, as evidenced by increased expression of gastrointestinal differentiation marker cytokeratin-20 (CK-20) [29] and an increase in alkaline phosphatase activity [30]. This evidence concerns the gene KRT20 and malignant colon neoplasm.