Furthermore, Dkk1 overexpression in HCT-116 colon cancer cell line resulted in restoration of the epithelial phenotype, decreased expression of Snail and Twist, two transcription factors considered to be key regulators of EMT [37], and decreased expression of colon CSCs markers (such as CD133 and leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5)). This evidence concerns the gene LGR5 and colonic neoplasm.