Moreover, in highly metastatic basal-like MDA-MB-231 breast cancer cells, has been showed that metformin treatment dynamically suppresses the CD44+/CD24−/low CSCs phenotype via transcriptional repression of the EMT machinery including transforming growth factor-β1, ZEB, Twist, and SLUG (Snail2) [75]. The gene discussed is SNAI2; the disease is breast carcinoma.