As confirmatory studies to examine if mitochondrial respiratory function is affected by expression of the mutant transgenes in our AD mice, we created a construct containing the same mutant APP(swe) and PS1(ΔE9) DNA (as the animals) with a fluorescent gene enhanced yellow fluorescent protein (EYFP) possessing a mitochondrial targeting sequence and a tetracycline response element. Here, PSEN1 is linked to Alzheimer disease.