Herein, we report further evaluation of the potential utility of SDC-1 as a diagnostic and prognostic biomarker in BCa by analysis of a large diverse test cohort through enzyme-linked immunosorbent assay (ELISA), and the investigation of SDC-1 protein expression patterns in bladder tumors through immunohistochemical (IHC) analysis of archival tissue specimens. Here, SDC1 is linked to urinary bladder neoplasm.