In this retrospective study, age, years of infertility, cervicitis, urinaryluteinizing hormone (LH) surge, insemination number, uterus position, endometrial thickness andmorphology, maximal follicle diameter, and the number of dominant follicles on the day of humanchorionic gonadotropin (HCG) administration were retrospectively analyzed in 501 women whounderwent their first ICI cycle using cryopreserved donor spermatozoa. This evidence concerns the gene PLOD1 and Infertility.