ENPEP and infection: As expected, 2APro strongly inhibited synthesis of both mature viral proteins (Ca-p24 and MA-p17) in cells supernatants and polyprotein precursors (Gag-p55 and Env-gp160) in cell lysates, at the highest proportions of 2APro (1∶3 and 1∶1) during the first 48 hours of infection (Figures 5D and E, respectively).