Furthermore, the MCF-7 breast cancer cell line, which has previously been used as a model in transfection experiments aiming at the functions of HMG proteins [37], [38], was transiently transfected with a eukaryotic expression vector encoding for wild-type HMGA2 to evaluate whether PLAG1 can be transcriptionally activated by HMGA2. Here, HMGA2 is linked to breast cancer.