Immunofluorescence staining showed that, compared with parental lung cancer cells, MSC-lung cancer hybrids exhibited a strong reduction in protein expression of E-cadherin and pancytokeratins as well as significantly increased expression of vimentin, α-SMA and fibronectin in all three NSCLC cell lines (Figures 3A–E). This evidence concerns the gene ACTA1 and non-small cell lung carcinoma.