Seventy-two-hour Annexin V apoptosis assays as well as ninety-six-hour TUNEL apoptosis assays involving three melanoma cell lines showed an increasing induction of apoptosis at higher doses of IFN-β; no increase in apoptosis occurred with IFN-α2, even at the highest dose (1000 units/mL), in any of the three cell lines [44]. This evidence concerns the gene IFNA2 and melanoma.