Seventy-two-hour Annexin V apoptosis assays as well as ninety-six-hour TUNEL apoptosis assays involving three melanoma cell lines showed an increasing induction of apoptosis at higher doses of IFN-β; no increase in apoptosis occurred with IFN-α2, even at the highest dose (1000 units/mL), in any of the three cell lines [44]. The gene discussed is IFNB1; the disease is melanoma.