For instance, 90 per cent of ARACNe-inferred and experimentally tested MYC targets in human B cells were validated as directly regulated by MYC [6], validation of targets in high-grade human glioma confirmed 40 out of 50 tested interactions for the transcription factors C/EBPβ, Stat3, RUNX1 and BHLHB2 [12], and the algorithm could correctly dissect a large number of transcriptional interactions involved in the synergistic control of germinal centre B cell proliferative programmes by FOXM1 and MYB [14,23,24]. The gene discussed is MYC; the disease is glioma.