To test whether Smac mimetics can also enhance TRAIL-mediated programmed necrosis, we incubated a set of five arbitrarily selected sensitive tumor cell lines (U-937, Mz-Cha-1, BxPC-3, HT-29 and Panc89; resistant KNS-62 cells were included as control) with TRAIL/zVAD/CHX (or TNF/zVAD/CHX) in the presence of the Smac mimetic birinapant. This evidence concerns the gene TNF and neoplasm.