Although calpain and caspase 3, components of the apoptotic signaling pathway, were activated by treatment with 5 μM β-lapachone for 0–9 h (Figure S1B), as shown in Supplementary Figure 2, caspases and calpain were not involved in the lung cancer cell death induced by β-lapachone, as 1 h pretreatment with the pan caspase inhibitor zVAD or the calpain inhibitor ALLM or ALLN (all from Sigma) did not inhibit the effect (Figure S2). Here, CASP3 is linked to lung carcinoma.