A similar profile was observed when TrkB-mediated calcium mobilization in TrkB-NFAT-bla CHO-K1 cells (FLIPR-based assay; data not shown) and TrkB-mediated AKT phosphorylation in human neuroblastoma SH-SY5Y cells were measured (MSD-based assay; Figure S1). The gene discussed is AKT1; the disease is neuroblastoma.