Therefore, in this study, we will further investigate the alterations of individual endothelial function-associated factors like ET-1, CGRP, VCAM, ICAM-1 and P-selectin in the susceptible brain regions of CVD such as frontal and temporal cerebral cortex, basal ganglia and thalamus (including the hypothalamus) in a rat model of T2DM. The gene discussed is EDN1; the disease is type 2 diabetes mellitus.