Our results are as follows: (1) ethyl pyruvate attenuate Con A-induced autoimmune hepatitis in Balb/C mice; (2) ethyl pyruvate decreases the TNF-α, IL-2, IL-6, IL-1β, and HMGB1 expression in vivo; (3) ethyl pyruvate may inhibit the release of HMGB1 through modulation of NF-κB signal pathway. The gene discussed is TNF; the disease is autoimmune hepatitis.