To characterize phenotypic changes caused by the mutated GNAS in pancreatic ductal cells, we employed HPDE cells (an immortalized cell line derived from healthy pancreatic duct epithelial cells) and pancreatic cancer cell lines (PK-8, PCI-35, and MIA PaCa-2) carrying KRAS mutations. The gene discussed is GNAS; the disease is pancreatic neoplasm.