Therefore, we aimed to examine the direct effect of the IkkαAA/AA knock-in mutation on canonical NF-κB activity in the context of atherosclerosis by studying the DNA binding capacity of p65 in nuclear extracts of IkkαAA/AAApoe−/− vs Ikkα+/+Apoe−/− BM-derived macrophages in vitro. Here, APOE is linked to atherosclerosis.