NES and neoplasm: As shown in Figure 4B, nestin knockdown in tumor cells resulted in prominent decrease in colony-forming ability (from 23% to 11.8% and 12.3% for shRNA1 and shRNA2 in A549 cells, and from 39.6% to 20.9% and 22.8% for shRNA1 and shRNA2 in A549 cells, respectively).