SOD1 and amyotrophic lateral sclerosis: To ensure that the WN1316 did not alter the expression of the human SOD1 transgene in ALS(SOD1H46R) mice, Western blot analysis using tissue extracts from the lumbar spinal cord in vehicle-treated and 10 μg/kg WN1316-treated groups and age-matched non-transgenic (non-Tg) littermates at 21–22 weeks of age was performed.