To analyze the impact of WN1316 on glial cell activation at sites of motor neuron loss in ALS(SOD1H46R) mice, immunostaining with anti-glial fibrillary acidic protein (GFAP) (for astrocytes) and anti-ionized calcium binding adaptor molecule 1 (Iba-1) (for activated microglia) antibodies using lumbar sections (L4–L5) was performed. Here, GFAP is linked to amyotrophic lateral sclerosis.