At 21–22 weeks of age, marked immunoreactivities both for GFAP and Iba-1 were observed in lumbar spinal cord anterior horn of vehicle-treated ALS(SOD1H46R) mice (Figure 6A, b and e), but not age-matched non-Tg (Figure 6A, a and d). Here, AIF1 is linked to amyotrophic lateral sclerosis.