At 21–22 weeks of age, the vehicle-treated ALS(SOD1H46R) mouse showed a massive loss of ChAT-positive motor neurons (Figure 5B) compared with non-Tg littermates (Figure 5A), whereas ChAT-positive motor neurons of 10 μg/kg WN1316-treated ALS(SOD1H46R) mouse were relatively spared (Figure 5C). This evidence concerns the gene CHAT and amyotrophic lateral sclerosis.