Expression of GATA4 and GATA5 was frequently lost while GATA6 was partially maintained at both mRNA (2−△Ct, mean 0.0006, 0.0001 and 0.0009, respectively, Fig. 1A) and protein levels (7/9, 5/9 and 2/9 negative, Fig. 1C) in hepatic and HCC cell lines. This evidence concerns the gene GATA4 and hepatocellular carcinoma.