Our results also showed that expression of GATA4/5 was frequently lost while GATA6 was retained in majorities of hepatic/HCC cell lines at both mRNA and protein levels (Fig. 1A and D), which theoretically supported others’ result showing frequent hypermethylation of GATA4/5 rather than GATA6 in carcinoma cell lines and tissues [14], [18], [41]. This evidence concerns the gene GATA6 and hepatocellular carcinoma.