In this study, the subgroup analyses showed that high serum AFP-L3% levels was also significantly related with OS and DFS in HCC treated by surgical resection (p = 0.0004, p = 0.0004, respectively), RFA (p<0.0001, p = 0.02, respectively), or multiple treatment (p<0.00001, p = 0.0004, respectively). Here, AFP is linked to hepatocellular carcinoma.