We found that the prognostic effects of high pre-treatment serum AFP-L3% on OS and DFS in HCC patients were similar for different treatment methods, between HBV infection and HCV infection, between highly sensitive and conventional AFP-L3 detection method, and between high AFP concentration and low AFP concentration (x2 p-value for subgroup differences >0.05). The gene discussed is AFP; the disease is hepatocellular carcinoma.