The well demarcated areas of no or low expression of GAL-1 is increased as the time of ischemia increases which mean that as time proceed dying cells will stop expression of GAL-1 in the area supplied by LAD, while survived cells in the ischemic zone, which are seen at the periphery of infarction zone, are showing high expression of GAL-1; which might explain the absence of statistical significance at 60 minutes, 4-hour and 24-hour post MI time points when compared to corresponding sham operated control groups. Here, LGALS1 is linked to ischemia.