To characterize the effect of DZ-50 on tight junctions (TJ) the co-localization of ZO-1 and Claudin-11, two proteins essential for these intercellular interactions, was assessed using fluorescent confocal microscopy in two different human prostate cancer cell lines, PC-3 and DU-145 (Fig. 3, panels A and B respectively). This evidence concerns the gene CLDN11 and Familial prostate cancer.