Here we demonstrate that i) treatment of L1CAM positive EC cells with DNA demethylating agents lead to rapid loss of L1CAM protein and mRNA expression; ii) miR-34a is a putative binderte the L1CAM -3′UTR; iii) manipulation of miR-34a levels can affect L1CAM expression levels; iv) an inverse correlation between the L1CAM and miR-34a expression was noted in EC cell lines and primary tumor tissues. This evidence concerns the gene L1CAM and neoplasm.