In contrast to the SRF/Mkl1 target genes that were predominantly associated with tumors classified as normal-like and with lower histological grades (1 and 2) (Figure 5A), elevated expression of SAP-dependent genes was associated with extremely high significance (P < 0.00001) with typical high-proliferative poor outcome classes in breast cancer, such as basal-like, HER2-enriched, luminal B, ER-negative and histological grade 3 tumors (Figure 5B). The gene discussed is ESR1; the disease is breast cancer.