To determine the level of Rae-1 expression on the murine cancer cell lines CT26, B16F10, LLC, TC1, and K7M3, we incubated cells with the 52A anti–Rae-1 mAb and then fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG and performed flow cytometry. The gene discussed is RAE1; the disease is cancer.