Osteocalcin could not be detected in patients with gingivitis [34].” Later in the year of 1994, Nakashima et al. reported “significant GCF osteocalcin levels from periodontitis and gingivitis patients [36].” “On evaluation of a combination of the biochemical markers osteocalcin, collagenase, prostaglandin E2, α2-macro-globulin, elastase, and alkaline phosphatase, increased diagnostic sensitivity and specificity values of 80% and 91%, respectively, were reported” by Nakashima et al. in 1996 [35]. The gene discussed is BGLAP; the disease is periodontitis.