Figure 4B confirms that this curve accurately predicts the observed purity for MCF7 and H1650 cells. To further confirm specificity of the EpCAM capture system, recovery of two EpCAM negative cell lines were tested on the platform at a spike in density of 10,000 cells/mL. Average recovery of both the Daudi, a B cell lymphoma, and the Jurkat, a T cell leukemia, cell line was less than 0.025%±0.3% (Table S2). This evidence concerns the gene EPCAM and B-cell non-Hodgkin lymphoma.