On the other hand, the levels of urinary KIM-1, a key molecule for early diagnosis of AKI [17], surged at 20 h, but did increase significantly at 4 and 8 h after IRI (Sham, 1.7±0.6 ng/ml; IRI 4, 1.3±0.5 ng/ml; IRI 8, 0.4±0.2 ng/ml; IRI 20, 37.8±1.1 ng/ml; IRI 40, 11.4±4.3 ng/ml) [F(4, 23) = 81.45, P<0.0001] (Fig. 6F). The gene discussed is HAVCR1; the disease is acute kidney injury.