Although high intensity electroacupuncture (10 mA, 64 Hz) induced higher expression of PGC-1α, low intensity electroacupuncture (2 mA, 8 Hz) was selected for this study to match manual acupuncture operation and maintain rats in a conscious and comfortable states in which rats is under quiet and happy state without anxiety and pain during whole experiments. Here, PPARGC1A is linked to Anxiety.