A total of 59 amplicons, including for the control SEPT9 and TMEFF2 genes, were prepared from DNA of each of 10 CRC and matched non-neoplastic tissues, as well as controls of pooled wbc DNA from individuals without cancer, fully methylated DNA (CpGenomeTM) and a 50:50 mix of wbc and fully methylated DNAs. Here, TMEFF2 is linked to cancer.