Interestingly, in primary cultured rat hepatocytes, metformin affected neither fatty acid oxidation nor triglyceride synthesis (Fulgencio et al. 2001), yet in an in vivo model of colon (Algire et al. 2010) and hepatocellular carcinoma (HCC) (Bhalla et al. 2012) with circulating cholesterol, metformin readily decreased FAS expression. Here, FAS is linked to hepatocellular carcinoma.