In our study, the risk of ALL in MTHFR 1298 AC genotype carriers was 2.084-fold that of the 1298AA genotype carriers (OR = 2.084, 95% CI: 1.131 - 3.841, P = 0.018), and the OR of the MTHFR A1298C genotype for AC + CC between patients and controls was 2.100 (95% CI, 1.149 - 3.837; P = 0.015). The gene discussed is MTHFR; the disease is acute lymphoblastic leukemia.